Auxin-Induced Water Uptake by Avena Coleoptile Sections.

Auxin-induced Changes in Avena Coleoptile Cell Wall Composition.

Sugar and uronic acid residues were derived from wall polysaccharides of oat (Avena sativa, var. Victory) coleoptiles by means of 2 N trifluoroacetic acid, 72% sulfuric acid, or enzymic hydrolysis. The products of hydrolysis were reduced and acetylated to form alditol acetates which were analyzed using gas chromatography. Time-course studies of auxin-promoted changes in various wall fractions i...

Improved Avena Coleoptile Elongation Test for Auxin'

Sum rv01,1f. A mo(lifie(l )rocedlure is presente(d for the bioassay of aulxin lusilng -Iv'eno coleoptile segments. The modifications introduicedl restult in a substantial improvement of the commonly uised( coleoptile elongatioil tests. The proposed test retains the simplicity of physical reqillremeints and of manipulatioIn characteristic of all elongatioln tests. The maiin featuire of the propo...

Steady state growth of avena coleoptile sections in high auxin concentrations.

Rapid Inhibition of Auxin-induced Elongation of Avena Coleoptile Segments by Cordycepin.

The effects of cordycepin (3'-deoxyadenosine), an RNA synthesis inhibitor, on auxin-induced elongation in Avena coleoptile segments were studied with a position-sensing transducer. Cordycepin rapidly inhibited auxin-stimulated growth in the coleoptile segments whether added before, at the same time as, or after, the 2 mum auxin treatment. Midcourse additions of 100, 50, and 25 mug/ml cordycepin...

Effect of pH and Auxin on Chloride Uptake into Avena Coleoptile Cells.

The effect of pH on (36)Cl(-) movement into coleoptile cells (Avena sativa L. cv. Garry) was investigated and compared with effects of indoleacetic acid. (36)Cl(-) uptake, but not efflux, is stimulated when coleoptile sections are placed in media adjusted to pH levels from 5 to 3 after a preincubation period at pH 6.5. The enhancement is seen within 2 minutes, is not correlated with growth, and...